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Year:
2002
| Volume: 10
| Issue: 1
| Pages: 19-23
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Original Article |
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EFFECTS OF IMMUNOREGULATORY DRUGS OF HUMAN PERIPHERAL BLOOD T LYMPHOCYTES FUNCTION IN VITRO
Marija HATZISTILIANOU, Soultana HITOGLOU, Despina GOUGOUSTAMOU, Alexandros KOTSIS, Athanasios KALLINDERES, Fanni ATHANASSIADOU, Dorothea CATRIU |
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DOI:
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Abstract: |
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Background: The purpose of the study was to evaluate the mode of action of different immunoregulatory drugs in lymphocyte proliferation and activation.
Methods: The drugs studied were prednisolone (PRED), cyclosporin A (CsA), the recombination of PRED and CsA, L-asparaginase and cytosine-arabinose (ara-C). Peripheral blood lymphocytes from normal blood donors were stimulated by phytohemagglutinin (PHA). Lymphocytes proliferation and activation were determined by tritiated thymidine ([3H] TdR) incorporation, secretion of interleukin-2, level of soluble interleukin-2 receptors in the supernatant of the culture medium, and immunophenotyping analysis of T lymphocyte subsets.
Results: Among PRED, CsA and their combination, the strongest inhibition of cell proliferation was induced by PRED while L-asparaginase and ara-C inhibited PHA-stimulated T cells proliferation in concentration and time dependent manner. Among PRED, CsA and their combination, CsA induced the greatest inhibition of IL-2 production. All the immunoregulatory drugs inhibited lymphocyte proliferation and expression of activation antigens.
Conclusion: The immunoregulatory drugs inhibit both lymphocyte proliferation and activation, but in a different way. |
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Key words:
T Lymphocytes; Prednisolone; Cyclosporine; Cytarabine; Asparaginase |
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