Founded in 1993
  Year: 2002 | Volume: 10 | Issue: 2 | Pages: 55-60
  Original Article
  IN VIVO MONITORING OF THERAPY-INDUCED APOPTOTIC PROCESS IN PATIENTS WITH CHRONIC LYMPHOCYTIC LEUKEMIA AND ACUTE NON-LYMPHOBLASTIC LEUKEMIA
Goran BRAJUSKOVIC, Slobodan MARJANOVIC, Andjelija SKARO-MILIC
  DOI:
  Abstract:
  Background: The level of spontaneous apoptosis, the maximal response by apoptosis and the time when maximal apoptotic response is established are significant prognostic parameters that correlate with the therapeutic response of patients with malignant diseases.
Methods: Our study included 12 patients with B-CLL (I group of 7 patients) and ANLL (II group of 5 patients). Detection of apoptotic parameters on semifine section of peripheral blood was performed. Bcl 2 and mutated p53 expression were analyzed using the monoclonal antibodies by APAAP techniques.
Results: In our work, the established levels of spontaneous and therapy induced apoptosis correlate with clinical response of patients. All patients were bcl 2 strongly positive. Expression of p53 protein was not detected in these patients. The ultra structural analysis of the malignant cells has shown that a great number of cells react to antineoplastic agents' effect either by activating the cell response to stress or by apoptosis.
Conclusion: The results of our pilot study have shown that the established levels of spontaneous and therapy - induced apoptosis are in correlation with the clinical response of the patients to the applied therapy and that the measurable apoptotic parameters can represent prognostic parameters in treatment of hematologic neoplasms.
  Key words: Apoptosis; Antineoplastic Agents; Leukemia; Gene Expression; Genes, bcl-2; Genes, p53
  Read full text in PDF [Full Text]
Next article

Previous article

Table of contents

Browse all Volumes

Search all Volumes
By keywords
By authors

  Search AoO for:
 

  Related articles in AoO:
 
About Journal | Editorial Board | Editorial Policy | Instructions for Authors | Open Access | Advertising | Payed issues | Article Submission Charge | Contact
Founder and owner: Oncology Institute of Vojvodina, Serbia
Publisher: Oncology Institute of Vojvodina
Co-publisher: Faculty of Medicine, University of Novi Sad
Online since 1997 (Abstracts only); 2000 (Abstracts and Full text)
ISSN: 0354-7310 eISSN: 1450-9520