Founded in 1993
  Year: 2003 | Volume: 11 | Issue: 1 | Pages: 1-4
  Original Article
  EFFECT OF INTRATUMORAL APPLICATION OF METHOTREXATE IN VIVO ON FREQUENCY OF MICRONUCLEI IN PERIPHERAL BLOOD LYMPHOCYTES
Tanja NOVAKOVIC, Olivera MILOSEVIC-DJORDJEVIC, Darko GRUJICIC, Dragoslav MARINKOVIC, Slobodan JANKOVIC, Slobodan ARSENIJEVIC
  DOI:
  Abstract:
  Background: Methotrexate is a cytostatic drug belonging to the group of antimetabolites; it is an antagonist of folic acid with an intensive application in gynecology practice.
Methods: The frequency of micronuclei (MN) in peripheral blood lymphocytes of 30 patients with myoma uteri was analyzed before and after intratumoral application of methotrexate (MTX) in total dose from 50 to 115 mg. Analysis of micronuclei was performed by the application of cytokinesis-block technique (CB).
Results: Average frequency of MN in lymphocytes of patients before the therapy was 4.60.4 MN/1000 analyzed cells. After the completion of therapy with six separate doses during six consecutive menstrual average frequency of MN increased 1.5 times (7.00.6) in comparison to control frequency before the therapy. Statistically signifi- cant difference (p<0.001) was established by the Student t test.
Conclusion: Methotrexate intratumoral treatment of the myoma uteri significantly increased micronuclei in peripheral blood lymphocytes. er of specialists.
  Key words: Methotrexate; Injections, Intralesional; Myoma; Uterine Neoplasms; Lymphocytes; Micronuclei
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Founder, owner and publisher: Oncology Institute of Vojvodina, Serbia
Online since 1997 (Abstracts only); 2000 (Abstracts and Full text)
ISSN: 0354-7310 eISSN: 1450-9520