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Year:
2004
| Volume: 12
| Issue: 4
| Pages: 200-202
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Original Article |
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INCIDENCE OF MICRONUCLEI IN PREGNANT WOMEN AND CORD BLOOD SAMPLES BEFORE AND AFTER THE BOMBING OF SERBIA
Stankovic M, Joksic G, Guc-Scekic M. |
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DOI:
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Abstract: |
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Background: This study provides the data regarding monitoring of population using CB-micronuclei assay in the period 1995-2001 in Serbia. The target groups consisted of 45 pregnant women of mean age 34.3(6.56) years, unaware of being exposed to chemicals drugs or other substances and undergoing cordocentesis. The incidence of micronuclei (MN) in peripheral blood lymphocytes and in fetal cord blood lymphocytes was analyzed.
Methods: The study was carried out on cultures of PHA-stimulated blood lymphocytes. Three drops of blood samples ware added into 5ml RPMI-1640 (Gibco) medium supplemented with 15% of calf serum and PHA (Gibco, 2.5µg/ml). For micronuclei preparation the cytokinesis block method was used (Fenech et al., 1993).
Results: The results of the study showed that in the year 1995, the incidence of micronuclei in pregnant women was 9.61(3.26) per 1000 binucleated (BN) cells, and 3.74(1.60) in cord blood samples per 1000 BN cells, respectively. In 2000 the incidence of micronuclei in study group was 28.26(7.87) per 1000 BN cells, and in cord blood samples 22.22(5.63) per 1000 BN cells. One year later (2001) the incidence of micronuclei in pregnant woman slightly decreased and reached the value of 26.98(4.50), while in cord blood it slightly increased up to 26.58(6.85) per 1000 BN cells.
Conclusion: The monitoring data obtained in this study have shown significantly increase of micronuclei (2- to 3-fold) in study groups in 2000 and 2001. |
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Key words:
Pregnancy; Fetal Blood; Micronuclei; Lymphocytes; Radiation Effects; War; Yugoslavia |
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