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Year:
2009
| Volume: 17
| Issue: 1-2
| Pages: 3-6
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Article |
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INFLUENCE OF AFLATOXIN B1 ON MRNA LEVELS OF ACUTE-PHASE PROTEINS AND ONCOPROTEINS IN ALBINO RAT LIVER
Nataša J. Strelic, Zvonko M. Magic, Nataša V. Trutic, Koviljka V. Krtolica |
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DOI:
10.2298/AOO0902003S |
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Abstract: |
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Background: The effect of aflatoxin B1 (AFB1) administration on expression of genes coding for acute-phase proteins, and nuclear protooncogenes c-fos and c-jun, and alpha-fetoprotein gene has been studied in rats.
Methods: AFB1 was administered to male Albino Oxford (AO) rats as a single intraperitoneal dose (1 mg/kg body weight). The expression of genes for albumin, cystein protease inhibitor, fibrinogen, haptoglobin, a1-acid glycoprotein and for c-Fos, c-Jun and alpha-fetoprotein in rat liver was measured by Northern hybridization.
Results: The mild increase in the levels of mRNA for acute-phase proteins after AFB1 administration was observed during the first 24 hours. The exceptions were the mRNA levels in liver for cystein protease inhibitor, which were 50%, decreased as compared to the control values. In addition, mild increase of the expression of c-fos protooncogene with two peaks were noted at three (1.3 fold) and 72 hours (1.5 fold) after injection AFB1 to rats. The expression of nuclear protooncogene c-jun at 1 hour and 72 hour after acute poisoning was 2.6 fold and 3.7 fold increased as compared to control values, respectively. The mRNA levels in liver for the alpha-fetoprotein reached a maximum at 1 hour after AFB1 injection and it was 1.8 times higher than the levels in the livers of nontreated animals.
Conclusion: Single administration of AFB1 induced increased transcription of c-jun and c-fos genes while typical acute-phase response was not found. |
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Key words:
Aflatoxin B1; Acute-Phase Reaction; Oncogenes; Gene Expression; Acute-Phase Proteins; Rats |
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