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Year:
2000
| Volume: 8
| Issue: 3
| Pages: 99-104
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Original Article |
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THE ALTERED ACTIVATION STATE OF MACROPHAGES ISOLATED FROM ASCITIC FLUID OF PATIENTS WITH PERITONEAL CARCINOMATOSIS
Dejan BASKIC, Ljubisa ACIMOVIC, Goran SAMARDZIC, Predrag DJURDJEVIC, Aleksandar DJUKIC, Nebojsa N. ARSENIJEVIC |
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DOI:
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Abstract: |
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Background: Cells of the monocyte/macrophage (Mo/Mph) lineage are capable of efficient killing of human nucleated tumor cells and this activity represents an important mechanism of host anti-tumor defense. However, in many cases developing neoplasms appear to be capable of impairing steps in this complex mechanisms as a means of avoiding immune destruction. The aim of this study was to investigate functional properties (phagocytosis and nitric oxide production) of cancer patient's mononuclear phagocytes. Materials and Methods: Mononuclear phagocytes used in this study were obtained following the plastic adherence technique. Nitric oxide production, measured by the Griees reaction, and phagocytosis test were used to quantify the monocyte/macrophage functions in 10 patients with peritoneal carcinomatosis. Results: Our result showed that phagocytic activity of peripheral blood monocytes (PBMo) was not altered, whereas tumor-associated macrophages (TAM) fromascitic fluid had impaired phagocytic activity. In addition, crude supernatant from short-term cultures of the peritoneal cells obtained from a cancer patient with peritoneal carcinomatosis have inhibitory effect on Mo/Mph phagocyte function, suggesting that tumor derived soluble factors are responsible for TAM inhibition. Moreover, TAM displayed decreased capacity to produce and/or release nitric oxide (NO), whereas monocytes produce NO at levels comparable to those of corresponding controls. Conclusion: Taken together, these data suggest that in the mechanisms of tumor dependent down-regulation of host defense functional disability of TAM may play an important role. |
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Key words:
Monocytes; Macrophages-peritoneal; Nitric-oxide; Neoplasms; Phagocytosis |
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