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Year:
2001
| Volume: 9
| Issue: 1
| Pages: 9-12
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Original Article |
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THE INFLUENCE OF 8-Cl-cAMP ON TUMOR MARKER CA19.9 ON EXPERIMENTAL GLIOMA IN RATS
ljiljana ANDRIJEVIC |
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DOI:
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Abstract: |
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Background: Since the existing chemotherapeutics cannot control all neoplastic processes, there is a need for discovering of new, more efficient drugs for cancer treatment and developing of specific tumor markers which would enable earlier diagnosis and follow up of cancer. The aim of this paper was to investigate the influence of 8-Cl-cAMP on expression of CA19.9 tumor marker in C6 cell line, impalnted in Wistar rat's brain.
Methods: Determination of CA 19.9 concentration in sera and brain tissue supernatants has been performed by immunoenzyme assay. Experimental glioma was induced by injection of 4x109 C6 cells into Wistar rat's brain. 8-Cl-cAMP was administered intraperitoneally in a single (50mg/kg/day) and multiple doses (50mg/kg/day, 5 days).
Results: The referent values of CA 19.9 in brain tissue supernatant ranged from 2.63 to 10.83 U/ml. The rate of concentrations of CA 19.9 was determined at five time points (1, 7, 14, 21 and 31 day) after the tumor cell implantation. Application of multiple doses of 8-Cl-cAMP significantly decreased CA 19.9 concentration, by 84.03 percent, at the seventh day of post treatment.
Conclusion: Obtained results suggested that CA 19.9 might be used as a tumor marker for determination of 8-Cl-cAMP influence on experimental glioma in rats. |
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Key words:
Cyclic AMP; Glioma; Tumor Markers, Biological; CA-19-9 Antigen |
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