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Year:
2000
| Volume: 8
| Issue: 4
| Pages: 157-159
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Original Article |
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EFFECT OF IRRADIATION ON ENZYMES OF ANTIOXIDATIVE DEFENSE SYSTEM IN L929 CELL CULTURE IN THE PRESENCE OF alfa-TOCOPHEROL-ACETATE
Visnja BOGDANOVIC, Gordana BOGDANOVIC, Gordana GRUBOR-LAJSIC, Aleksandar RUDIC, Vladimir Vit. BALTIC |
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DOI:
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Abstract: |
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Background: Cell damage induced by ionising radiation occurs through direct and indirect effects. Vitamin E as a natural antioxidant protects against radiation-induced cell damage. Enzymes of antioxidative defense system - superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), play the main role in endogenous cell protection from oxidative damage. The aim of this study was to examine whether a presence of a-tocopherol-acetate has the influence on activity of SOD, GSH-Px and CAT in L929 cell culture after the irradiation.
Materials and Methods: Cell culture L929 samples were treated with a-tocopherol acetate (10-4M) during one hour and thereafter cells were irradiated with X-rays (25 Gy). Activity of SOD, GSH-Px and CAT was determined at 1, 24, 48, 72 and 96 hours after irradiation.
Results: Irradiation in applied dose induces decrease in endogenous SOD activity but no changes in CAT and GSH-Px activity of L929 cells were observed, at 96 h after irradiation. Activity of SOD was significantly increased (p1<0.05), CAT activity was significantly decreased (p1<0.05), while no significant difference was found in activity of GSH-Px in irradiated and a-tocopherol acetate pre-treated L929 cells, in comaprison to irradiated but unprotected cells.
Conclusion: A treatment of L929 cells with a-tocopherol acetate before irradiation protects SOD, has no effect on GSH-Px and induces decrease of CAT activity after 96 hours of irradiation. As radiation exposure itself results in changes of antioxidant enzyme activities, observed changes in the presence of a-tocopherol acetate were discussed with respect to proper balance of antioxidative defense required for radioprotection. |
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Key words:
Cell culture; Radiation; Alpha tocopherol-acetate; Antioxidative enzymes |
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