Founded in 1993
  Year: 2013 | Volume: 21 | Issue: 2 | Pages: 59-61
  Original Article
  CYTOTOXICITY OF SINGLE-WALLED CARBON NANOTUBES TO HUMAN LUNG CARCINOMA CELLS: THE INFLUENCE OF N-ACETYLCYSTEINE
Nikola Jojic, Vesna Kojic, Danijela Kojic, Karmen Stankov, Gordana Bogdanovic
  DOI: 10.2298/AOO1302059J
  Abstract:
  Background: Single-walled carbon nanotubes (SWCNTs) have been reported to induce cytotoxicity in different cell lines. Although the mechanisms underlying cytotoxicity are not fully understood, accumulation of reactive oxygen species (ROS) and oxidative damage is considered to be a likely contributing factor. Methods: Human lung carcinoma cells, A549, and human fetal lung fibroblasts, MRC-5 were used to assess the cytotoxicity of SWCNT in the presence and absence of a redox status regulator, N-acetylcysteine (NAC), via the MTT assay. Results: SWCNT induced a nearly three-fold greater loss of viability in A594 vs. MRC-5 cells at ?250 g/ml. SWCNT cytotoxicity at higher concentrations was similar for both cell lines, while NAC alone was non-toxic. The cytotoxicity of SWCNT (250 g/ml) in combination with NAC to A549 cells was significantly decreased at the lowest NAC concentration (1.5 g/ml), and was similar to NAC treatment alone at that concentration. Higher concentrations of NAC in combination with SWCNT (250 g/ml) resulted in increased cytotoxicity in both A549 and MRC-5 cells. Conclusion: A549 malignant lung cells are more susceptible to low concentrations of SWCNT vs. normal lung cells, and low concentrations of N-acetylcysteine appear to be cytoprotective, possibly due to its antioxidant properties.
  Key words: Nanotubes, Carbon; Acetylcysteine; Oxidative Stress; Cell Line, Tumor
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Founder, owner and publisher: Oncology Institute of Vojvodina, Serbia
Online since 1997 (Abstracts only); 2000 (Abstracts and Full text)
ISSN: 0354-7310 eISSN: 1450-9520