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Year:
1997
| Volume: 5
| Issue: 3
| Pages: 147-149
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Original Article |
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BIOLOGICAL ACTIVITY OF WATER-SOLUBLE FULLERENCE: C60(OH)24
Gordana BOGDANOVIC, Mirjana VOJINOVIC-MILORADOV, Vesna KOJIC, Aleksandar DJORDJEVIC, Janos CANADI, Djuro KORUGA, Vladimir Vit. BALTIC, Dunja TABS |
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DOI:
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Abstract: |
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Background: Fullerenes and their water-soluble derivatives have recently become a topic of interest in biomedicine. Here, we report on citotoxicity and antiproliferative activity of water-soluble derivative of fullerene C60 against five cell lines: K562, MCF7, Raji, L929 and BHK. Methods: The cells were continuously treated with derivative up to 24 or 48 h and citotoxicity and cellular survival were assessed by MTS/PMS and dye exclusion test. Results: Water-soluble fullerene derivative induce low growth-inhibition rate in cell lines except Raji, during 24 or 48 h. Growth-inhibition was transient and the cells, that had been previously treated with derivative in duration of 48 h, successfully survived; after 48 h of growth in fresh medium they achieved the control sample values, regardless the concentration of derivative and the type of cell line. Conclusion: The preliminary results of this study showed that generally low but transient growth-inhibition activity of water-soluble derivative C60 was obtained on the most of cell lines, regardless of their source and features. Raji cells appeared to be resistant to growth-inhibition activity of derivative in given experimental conditions. |
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Key words:
Fullerene C60; Cell lines; Cytotoxicity; Cell growth; Tetrazolium salts; MTS/PMS assay |
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