Founded in 1993
  Year: 1998 | Volume: 6 | Issue: 4 | Pages: 159-162
  Original Article
  TRANSFORMING GROWTH FACTOR - Beta1 AS A PROGNOSTIC BIOMARKER OF PROSTATE CARCINOMA
Vesna IVANOVIC, Miroslav DEMAJO, Olivera IVANISEVIC-MILOVANOVIC
  DOI:
  Abstract:
  Background: The etiology and biology of prostate carcinogenesis is mainly unknown. Recent results have indicated prognostic significance of Transforming Growth Factor-b1 (TGF-b1) in this malignancy. The main objective of the present study was to investigate the TGF-b1 changes in the blood specimens of patients with prostatic adenocarcinoma (CaP) relative to normal donors.
Methods: The TGF-b1 levels were measured in peripheral blood of 24 CaP patients and 20 normal donors, using a commercial TGF-b1 enzyme-linked immunosorbent assay. A specific protocol for preparation of platelet-poor plasma has been described. The plasma and serum were used as specimens in 18 and 6 CaP patients, respectively.
Results: Our data indicate striking qualitative and quantitative differences between the TGF-b1 levels in serum and plasma presumably due to platelet content, which should be eliminated early in the blood sample preparation. Our results favor the use of platelet-poor plasma rather than serum for direct TGF-b1 measurements by commercial immunoassays. We further demonstrate the increase in plasma TGF-b1 levels of CaP patients relative to normal, which did not correlate with Gleason grade, but correlated with the pathologic stage of the disease. The advanced CaP (Stage III/IV) patients exhibited highly significant increase in TGF-b1 levels relative to normal donors and Stage II patients.
Conclusion: These findings confirm our previous observation and provide a follow up evidence that TGF-b1 might be an early indicator of progression and metastasis. Our results are consistent with the use of plasma TGF-b1 as a biomarker to distinguish CaP patients who have high risk of developing subsequent invasion or metastasis.
  Key words: HUMAN PROSTATE CANCER; SERUM; PLASMA; PROGNOSTIC BIOMARKER; PROGRESSION
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Founder, owner and publisher: Oncology Institute of Vojvodina, Serbia
Online since 1997 (Abstracts only); 2000 (Abstracts and Full text)
ISSN: 0354-7310 eISSN: 1450-9520