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Year:
1999
| Volume: 7
| Issue: 3
| Pages: 101-104
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Original Article |
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DISCRIMINANT ANALYSIS OF KARYOMETRIC DIFFERENCES BETWEEN KERATOACANTHOMA AND SQUAMOUS CELL CARCINOMA
Dragan MIHAILOVIC, Milenko STANOJEVIC, Zaklina MIJOVIC, Zoran PESIC |
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DOI:
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Abstract: |
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Background: Keratoacanthoma is a fairly common, benign, usually self-resolving skin lesion. Because of similarities in clinical presentation and histology to squamous cell carcinoma, the diagnosis of keratoacanthoma can be challenging. The aim of this study was to estimate karyometric features in keratoacanthoma and squamous cell carcinoma.
Methods: At Institute of Pathology, University of Nip, formalin-fixed, paraffin-embedded skin biopsies from 16 cases of solitary keratoacanthoma and 22 cases of well-differentiated squamous cell carcinoma were retrieved from dermatopathology archives. Specimens were routinely stained with hematoxylin and eosin (HE), and analyzed by LUCIA M image analyzer. Nuclear area, volume of equivalent sphere, circularity, and integrated density were estimated after editing of binary image. Volume-weighted nuclear volume was estimated by point sampled nuclear intercept method.
Results: All variables were higher in keratoacanthoma; differences between nuclear volume and integrated density were statistically significant. Using stepwise linear discriminant function analysis, 90% of squamous cell carcinoma cases and 100% of keratoacanthoma cases were accurately classified, respectively. Nuclear volume acts as a major discriminant variable.
Conclusion: Nuclear size acts as a major discriminant variable and may be of value in the differential diagnosis of keratoacanthoma and squamous cell carcinomas in routine histopathology. |
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Key words:
Keratoacanthoma; Squamous cell carcinoma; Image analysis; Nuclear volume; Integrated density |
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