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Year:
2001
| Volume: 9
| Issue: 3
| Pages: 145-149
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Original Article |
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INFLUENCE OF TRANSITORY ANOXIA AND
REOXYGENATION ON GLUTATHIONE SYSTEM IN
INFLUENCE OF TRANSITORY ANOXIA AND
REOXYGENATION ON GLUTATHIONE SYSTEM IN
INFLUENCE OF TRANSITORY ANOXIA AND REOXYGENATION ON GLUTATHIONE SYSTEM IN MALIGNANT CELLS
Karmen STANKOV, Zoran KOVACEVIC, Katica BAJIN-KATIC |
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DOI:
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Abstract: |
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Background: It is well documented that repeated shorter hypoxia and ischemia result in adaptation of normal cells and tissues to decreased partial pressure of oxygen, and that they become more resistant to anaerobiosis. In malignant cells, this adaptation is incomparably better, indicating that these cells are often exposed to temporary lack of oxygen.The aim of our study was to elucidate the specificities in response of malignant cells to anoxia and reoxygenation, and the possibility of some metabolites to change sensitivity of malignant cells to anaerobiosis.
Methods: Concentrations of total glutathione (tGSH) and oxidised glutathione (GSSG) in Ehrlich ascites carcinoma (EAC) cells and AS-30D hepatoma (ASH) cells were determined by enzymatic method with glutathione reductase.
Results: Anaerobic incubation of EAC and ASH cells resulted in tGSH depletion and disturbance of GSH/GSSG ratio, while the presence of glutamine or glucose in medium resulted in preservation of cellular redox state. Reoxygenation of EAC and ASH cells resulted in regeneration of GSH content. The sensitivity and response of EAC cells to transitory anoxia and reoxygenation were different from ASH cells, which was emphasized by supplementation of N-acetylcysteine to EAC and iodoacetate to ASH.
Conclusion: Glutamine and glucose metabolisms in malignant cells significantly influence the sensitivity to anaerobiosis as well as redox and energy state of malignant cells. |
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Key words:
Anoxia; Oxygen; Glutathione; Tumor Cells, Cultured |
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