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STUDY
ON MUC2 AND MUC3AC - TUMOR ASSOCIATED MARKERS PRESENT IN MUCIONOUS
COLORECTAL ADENOCARCINOMA AND ITS PRECANCEROUS TUMORS
Katić
K1, Katić V1, Nagorni
A2, Krstić M1.
1Institute
of Pathology, Medical Faculty of Niš, Niš, Serbia and Montenegro
2Clinic
of Gastroenterology, Medical Faculty of Niš, Niš, Serbia and Montenegro
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ABSTRACT
Information
regarding the expression of colorectal mucins has accumulated during
the last decade. The proteins and their O-linked glycans were demonstrated
to represent tumor-associated markers in humans. Having in mind
that previous investigations revealed inconsistence on MUC2 and
MUC3AC in mucionous adenocarcinomas and that there are only a few
data on its histogenesis, we have undertaken comparative histological,
histochemical and immunocytochemical investigations on MUC2 and
MUC3AC in above pointed out lesions. We examined a consecutive series
of 50 patients with colorectal mucinous adenocarcinomas (10 patients),
serrated hyperplastic polyps, serrated adenomas, mixed polyps (30
patients), and villous adenomas (10 patients). Patients underwent
a curative surgical resection or endoscopic polypectomy. Formalin-fixed
paraffin-embedded tissues were cut and deparaffinized according
to standard histological techniques. Laboratory sections were stained
with HE, PAS, HID-AB, ph=2.5 and with imunohistochemical biotin-streptavidin-peroxidase
techniques, by using MoAbs MUC2 and MUC3AC (1:200). Histologically
serrated polyps (hyperplastic, mixed polyps, serrated adenomas),
villous adenomas and mucinous adenocarciomas were diagnosed. Histologically,
only the fields with goblet cells (not columnar cells) contained
intestinal-syalo mucin. Immunohistochemically, goblet cells in serrated
polyps, villous adenomas and mucinous adenocarcinomas expressed
MUC2 and MUC3AC strongly. The present study demonstrates the new
concept that serrated polyps, villous adenomas and mucinous adenocarcinomas
represent a histogenetic continuum, and that hyperplastic polyp
is not necessary an entirely innocuous lesion; by studying MUC phenotypes,
it may be possible to generate new lineage-based classification
of colorectal cancers that reflect the presence and level of function
of goblet cells versus columnar cells. |
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Keywords:
Mucionous adenocarcinomas, Goblet cells, MUC phenotype, Imunohistochemical
analysis |
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