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APPLICATION
OF SPECTROSCOPIC AND ELECTRO CHEMICAL METHODS IN MALIGNANT TUMORS
AND IN THE EVALUATION OF ANTI-TUMOR DRUG ACTIVITY
M.
Avramov Ivić1, S.
D. Petrović2,3, G.
Bogdanović4, M. Baltić4,
L. Aleksić4, V. Vit. Baltić4,
E. Kálmán5,
Z. Keresztes5
1ICTM-Institute
of Electrochemistry, University of Belgrade, Yugoslavia
2Faculty of Technology and Metallurgy, University
of Belgrade
3Hemofarm Group, Pharmaceutical industry,
Vršac, Yugoslavia
4Institute of Oncology, Sremska Kamenica,
Yugoslavia
5Institute of Chemistry of the Chemical
Research Center of the Hungarian Academy of Sciences, Budapest,
Hungary
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ABSTRACT
The aim of this study was to prove the existence of anti-tumor drug
- DNA adducts using AFM spectroscopy and cyclic voltammetry and
to introduce the UV-VIS spectroscopy in the analysis of malignant
effusions as a simple, fast and informative diagnostic method.Materials
used for analysis were: DNA samples of different malignant tissues
- untreated or treated with commonly used cytostatic drugs. AFM
spectroscopy as well as cyclic voltammetry was used in the analysis
of DNA samples and cytostatic drugs. In the analysis of the pleural
effusions, ascites and pericardial effusions UV-VIS spectroscopy
was used.Cyclic voltammetry of anti-tumor drugs (e.g. cis-platin
and Doxorubicin) gave a possibility of an exact determination of
active groups related to the cytostatic DNA complex formation. AFM
spectroscopy enabled us to see the shape as well as to estimate
a size of the particles, which were the consequence of the assumed
aggregation. Preliminary results indicated that aggregate formation
occurred in a case of DNA treated with cisplatin. The body liquids
were analyzed by UV-VIS spectroscopy and by this method malignant
from non-malignant effusions were differentiated in a way that malignant
origin of the fluid could be always recognized. The results showed
that a usage of cyclic voltammetry and AFM could have a significant
role in efforts to understand phenomena concerning anti-tumor drug's
activity. The UV- VIS spectroscopy could serve as a fast screening,
non-invasive diagnostic method to differentiate malignant from non-malignant
effusions. The introduction of above-mentioned methods as new, additional
diagnostic methods into common medical practice deserves to be evaluated.
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