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8th
International Inter University Scientific Meeting
Academy of Studenica
NEW
TRENDS IN DIAGNOSTICS AND THERAPY OF MALIGNANT TUMORS
Organizer:
Institute of Oncology Sremska Kamenica, Yugoslavia
Co-organizers: Institute
for Oncology and Radiology, Belgrade, Yugoslavia;
"Aristotel School", Thessaloniki, Greece
President: Prof.Dr.
Vladimir Vit. Baltić
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FULLEROL
AS A LIGAL AND RADICAL TRAPPER
A. Đorđević1,
J. Čanadanović-Brunet2, M. Vojinović-Miloradov1,
R. Kovačević1, S. Mirkov1,
N. Andrić1, G. Bogdanović3
1Faculty
of Sciences, University of Novi Sad, Yugoslavia
2Faculty of Technology, University of Novi
Sad, Yugoslavia
3Institute of Oncology Sremska Kamenica,
Yugoslavia
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ABSTRACT
Chemical
modification of hydrophobic fullerene C60 molecule yielded many
water-soluble adducts exhibiting the variety of biological activities.
In this paper the results of chemical investigation of fullerol
(polyhydroxy derivate of C60 (C60 (OH) 24) as a ligand for the iron
cations as well as a radical scavenger were presented. Polyhydroxylated
fullerene C60 water-soluble derivative, fullerol, C60(OH)24, as
well as fullerol-Fe+2 and Fe3+ complexes were synthetized and characterized
in the laboratories of the Chemistry Department, Faculty of Sciences,
University of Novi Sad. ESR spin "trapping" method was used to assay
radical scavenging activity of the fullerol.In water, fullerol formed
insoluble complex with Fe3+ and Fe2+. These complexes were thermally
unstable but were soluble in high alkaline medium. At the pH values
close to physiological pH, the complexes were very stable. This
paper presents the hypothetical structure of the fullerol-Fe3+ complex.
We also reported the experimental investigation of antioxidative
properties of fullerol C60(OH)24 in the chemical model system. Relative
intensity (RI) of ESR signals of PBN-OH (*-phenyl-N-t-butylnitrone-OH)
adduct, in the presence of 0.88 mmol/dm3 of fullerol, were decreased
by 82.2%. The intensity of the new signal was increased by time
while the intensity of the second basic signal of PBN-OH adduct
was decreased. Based on these spectra it can be proposed that the
new signal represents a radical of fullerol C60(OH)24-.. Mechanisms
of effects of fullerol in in vitro model systems could be explained
in two ways: either as a chelate, or as a hydroxy radicals scavenger.
Experimental proof of this hypothesis is in progress. |
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Keywords:
C60, fullerol: Antioxidant: ESR, .OH: Chelate |
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