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10th
International Inter University Scientific Meeting
Academy of Studenica
PERSPECTIVES
IN MELANOMA MANAGEMENT
& NANOTECHNOLOGY IN BIOMEDICINE
Organizers:
Institute of Oncology
Sremska Kamenica; Union of Cancer Prevention
Societies of Vojvodina, Novi Sad; Clinic of Oncology, Nis; Institute
for Oncology and Radiology of Serbia, Belgrade Center for Bioengineering,
Faculty of Mechanical Engineering, University of Belgrade
President:
Vladimir Baltic Vice-presidents: Zlata
Janjic, Radan Dzodic, Borislava Nikolin; Djuro Koruga
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ANTIOXIDATIVE
ACTIVITY AND HYPOTHETIC RADICAL MECHANISM OF FULLERENOL
Đorđević
A, *Čanadanović-Brunet J, Vojinović-Miloradov M, **Bogdanović
G.
University
of Novi Sad, Faculty of Sciences, Serbia and Montenegro
* University of Novi Sad , Faculty of Technology Sciences, Serbia
and Montenegro
** Institute of Oncology Sremska Kamenica, Serbia and Montenegro
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ABSTRACT
Polyhydroxylated
derivatives of C60 (fullerols, fullerenol)
of the general formula C60(OH)n
(n=10-26) belong to the group of water-soluble biologically active
compounds, extremely important for the application in biomedicine,
pharmacy, and cosmetics. In the last few years a large body of evidence
on diverse biological activity of water - soluble C60
derivatives has been reported. Recently, the use of water-soluble
fulleronols as superoxide anion free-radical scavengers (O2-.)
generated by in vitro xanthine and xanthine oxidize, has been reported.
Similar activities were also observed in trapping highly reactive
hydroxyl radicals (HO-) generated by
H2O2 in the presence
of Fe+2. Water-soluble
fullerene derivatives have been demonstrated to scavenge oxygen
radicals in vitro. Endohedral fullerenol (Hox@C82(OH)y,
Gd@C82(OH)n(NHCH2COOH)m;
Gd@C82(OH)n; Gd@C60(OH)n; Gd@C60(CHCOOH)4)
could be useful for therapeutic and diagnostic methods. As hydroxyl
free radicals are highly reactive, with relatively short half-lives,
the concentrations found in natural systems are usually inadequate
for direct detection by ESR spectroscopy. According to experimental
results in chemical model systems (Fenton reaction, and SNP), for
OH. radicals production, dose antioxidative dependence of fullerenol,
has been confirmed. At concentration of fullerenol of 0.88 mmol/dm3,
intensity of middle DMPO-hydroxyl radical adduct decreased for 82.2%.
The predicted mechanism of antioxidative effect of fullerenol in
chosen model systems is most probably of radical type (this was
confirmed by the wide, non- defined peak on ESR spectrum (Fenton's
reaction)). We think that we are dealing with more fullerenol radicals
(fullerenol radical cation C60(OH)20O2H2+.;
fullerenol radical C60(OH)19O2H2O.;
C60(OH)20O2H2
+ nOH.). |
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Keywords:
C60, fullerenol, water soluble, antioxidants, ESR, .OH,
mechanism
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