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10th
International Inter University Scientific Meeting
Academy of Studenica
PERSPECTIVES
IN MELANOMA MANAGEMENT
& NANOTECHNOLOGY IN BIOMEDICINE
Organizers:
Institute of Oncology
Sremska Kamenica; Union of Cancer Prevention
Societies of Vojvodina, Novi Sad; Clinic of Oncology, Nis; Institute
for Oncology and Radiology of Serbia, Belgrade Center for Bioengineering,
Faculty of Mechanical Engineering, University of Belgrade
President:
Vladimir Baltic Vice-presidents: Zlata
Janjic, Radan Dzodic, Borislava Nikolin; Djuro Koruga
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ANTIOXIDATIVE
ACTIVITY AND HYPOTHETIC RADICAL MECHANISM OF FULLERENOL
Đorđević A, *Čanadanović-Brunet J, Vojinović-Miloradov
M, **Bogdanović G.
University
of Novi Sad, Faculty of Sciences, Serbia and Montenegro
* University of Novi Sad, Faculty of Technology Sciences, Serbia
and Montenegro
** Institute of Oncology Sremska Kamenica, Serbia and Montenegro
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ABSTRACT
Background:
Biological properties of water-soluble [C60]
fullerene derivatives attracted attention of the researchers in
recent years, but there are insufficient data of their genotoxicity,
especially related to human material. The toxicity and mutagenicity
of this class of compounds were studied so far mostly by testing
their ability to induce mutations in prokaryotic genomes (Ames tests),
using considerably high concentrations of the substance. The groundwork
for this study was our recent in vitro investigations of the short-time
cytotoxic and cytostatic activities of fullerenol, at nanomolar
concentrations, on continuous human leukemia and solid tumor cell
lines and our findings of protective effect of the fullerenol, its
influence on growth, proliferation and cell cycle, and strong suppression
of antitumor drug induced cytotoxicity by this compound.
Methods: This study represents a genotoxic investigation
of polyhydroxylated [C60] fullerene derivative
- fullerenol C60(OH)22
on peripheral blood lymphocytes of healthy persons. Cells were seeded
in control and experimental cultures and stimulated to divide one
hour after fullerenol was added to experimental samples at final
concentration of 1 or 5 g/ml (several fold lower then concentrations
used in earlier mutagenicity tests). After incubation, we performed
a battery of classic cytogenetic genotoxicology tests of control
and fullerenol treated cells (chromosomal aberrations analysis,
sister-chromatid exchanges test, and micronucleus assay), in order
to find how it affects the exposed human genetic material.
Results: The fullerenol C60(OH)22,
administrated at nanomolar concentrations, did not induce detectable
chromosomal aberrations, but significantly reduced the exchanges
between sister chromatids in average for 10%, comparing to control.
The incidence of micronuclei in cells of the samples treated with
fullerenol was also reduced for nearly 40% in comparison to control,
while micronucleus distribution was shifted toward lower number
of micronuclei per binuclear cell.
Conclusion: This study showed the absence of genotoxicity
of investigated fullerenol, and the results suggest that it could
be useful compound for developing different models for genotoxic
investigations, even designing drugs, such those that diminish of
genotoxic strikes and outcomes. |
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Keywords:
Fullerene C60; Fullerene derivative; Fullerenol; Genotoxicity;
Cytogenetic tests |
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