Founded in 1993
  Year: 2000 | Volume: 8 | Issue: 3 | Pages: 99-104
  Original Article
  THE ALTERED ACTIVATION STATE OF MACROPHAGES ISOLATED FROM ASCITIC FLUID OF PATIENTS WITH PERITONEAL CARCINOMATOSIS
Dejan BASKIC, Ljubisa ACIMOVIC, Goran SAMARDZIC, Predrag DJURDJEVIC, Aleksandar DJUKIC, Nebojsa N. ARSENIJEVIC
  DOI:
  Abstract:
  Background: Cells of the monocyte/macrophage (Mo/Mph) lineage are capable of efficient killing of human nucleated tumor cells and this activity represents an important mechanism of host anti-tumor defense. However, in many cases developing neoplasms appear to be capable of impairing steps in this complex mechanisms as a means of avoiding immune destruction. The aim of this study was to investigate functional properties (phagocytosis and nitric oxide production) of cancer patient's mononuclear phagocytes.
Materials and Methods: Mononuclear phagocytes used in this study were obtained following the plastic adherence technique. Nitric oxide production, measured by the Griees reaction, and phagocytosis test were used to quantify the monocyte/macrophage functions in 10 patients with peritoneal carcinomatosis.
Results: Our result showed that phagocytic activity of peripheral blood monocytes (PBMo) was not altered, whereas tumor-associated macrophages (TAM) fromascitic fluid had impaired phagocytic activity. In addition, crude supernatant from short-term cultures of the peritoneal cells obtained from a cancer patient with peritoneal carcinomatosis have inhibitory effect on Mo/Mph phagocyte function, suggesting that tumor derived soluble factors are responsible for TAM inhibition. Moreover, TAM displayed decreased capacity to produce and/or release nitric oxide (NO), whereas monocytes produce NO at levels comparable to those of corresponding controls.
Conclusion: Taken together, these data suggest that in the mechanisms of tumor dependent down-regulation of host defense functional disability of TAM may play an important role.
  Key words: Monocytes; Macrophages-peritoneal; Nitric-oxide; Neoplasms; Phagocytosis
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Founder, owner and publisher: Oncology Institute of Vojvodina, Serbia
Online since 1997 (Abstracts only); 2000 (Abstracts and Full text)
ISSN: 0354-7310 eISSN: 1450-9520